Arsenicum Album 200, Can Ameliorate Genotoxicity Induced by Repeated Injections of Arsenic Trioxide

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Groundwater arsenic contamination has become a menacing
global problem. No drug is available until now to combat
chronic arsenic poisoning. To examine if a potentized homeopathic
remedy, Arsenicum Album-200, can effectively
combat chronic arsenic toxicity induced by repeated injections
of Arsenic trioxide in mice, the following experimental
design was adopted. Mice (Mus musculus) were injected
subcutaneously with 0.016% arsenic trioxide at the rate of
1 ml/100 g body weight, at an interval of 7 days until they
were killed at day 30, 60, 90 or 120 and were divided into
three groups: (i) one receiving a daily dose of Arsenicum
Album-200 through oral administration, (ii) one receiving the
same dose of diluted succussed alcohol (Alcohol-200) and (iii)
another receiving neither drug, nor succussed alcohol. The
remedy or the placebo, as the case may be, was fed from the
next day onwards after injection until the day before the next
injection, and the cycle was repeated until the mice were
killed. Two other control groups were also maintained: one
receiving only normal diet, and the other receiving normal
diet and succussed alcohol. Several toxicity assays, such as
cytogenetical (chromosome aberrations, micronuclei, mitotic
index, sperm head anomaly) and biochemical (acid and
alkaline phosphatases, lipid peroxidation), were periodically
made. Compared with controls, the drug fed mice showed
reduced toxicity at statistically significant levels in respect of
all the parameters studied, thereby indicating protective
potentials of the homeopathic drug against chronic arsenic


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